Refeyn, the corporate behind pioneering mass photometry applied sciences, has taken one other step to guarantee mass photometry is an easier, extra environment friendly bioanalytics instrument with the discharge of a robust growth of its consumables portfolio. The introduction of next-generation microfluidic chips and enhancements to Refeyn’s microfluidic system – in addition to a brand new protein calibration normal – will save customers time, whereas making certain reproducibility throughout experiments and labs, and supporting a broader vary of purposes.
To reinforce the speedy dilution of extremely concentrated samples and enhance perception into low-affinity and transient biomolecular interactions, a number of updates have been made to Refeyn’s in style MassFluidix® HC microfluidics system. A key addition to this method is a 2nd technology microfluidic chip which now permits mass photometry measurements in 5 channels and options even sooner pattern dilution – in below 37 milliseconds. Utilization is simplified with a channel redesign that removes the necessity for a bubble lure and introduces luer connectors that allow straightforward connection of tubing to the chip. These ready-to-use chips are additionally now individually packaged, permitting prospects to open simply one after the other as wanted.
Additional enhancing ease-of-use of the MassFluidix HC, software program integration now implies that the speedy dilution system may be readily operated straight through Refeyn’s AcquireMP mass photometer management software program. A brand new computerized microfluidic chip channel detection function inside this software program additionally simplifies workflows by making certain right positioning of the chip for every measurement.
The upgraded system’s speedy dilution permits the characterization of samples at excessive focus. That is significantly helpful as a high quality verify for methods that require larger concentrations, similar to cryoEM and crystallography, in addition to for detecting and figuring out stoichiometry for weak affinity protein-protein interactions. This has implications for historically difficult-to-characterize pattern sorts, similar to PROTACs and multi-component complexes; along with extra widespread purposes, similar to antibody-receptor binding, and as an orthogonal approach to different binding characterization methods.
Moreover, a brand new mass calibrant joins Refeyn’s portfolio of calibration requirements, which guarantee most precision of mass photometry measurements and minimise trouble for patrons. Easy to arrange, Refeyn’s MassFerence® mass calibration normal vary offers standardized references, rising replicability throughout experiments, devices and laboratories. The brand new MassFerence® P1 protein normal is launched to be used with Refeyn’s TwoMP and OneMP mass photometers for calibrating measurements of proteins starting from 90 to 1000 kDa.
Commenting on Refeyn’s rising mass photometry consumables vary, Gerry Mackay, CEO, Refeyn, mentioned, “These new merchandise mirror our dedication to responding to buyer wants and dealing to offer the very best expertise for all customers of mass photometry applied sciences. With their introduction, we intention to optimize the effectivity and high quality of mass photometry experiments, and making mass photometry helpful for a wider vary of samples.”
We’ve listened to our prospects and made many adjustments and additions to go well with their necessities. Through the use of consumables that we’ve developed particularly for Refeyn devices, our prospects can profit from hassle-free measurements, with much less time wanted for pattern prep and extra confidence of their mass photometry biomolecular evaluation outcomes.”
Camille Hetez, Director of Consumables, Refeyn
